About WetLab Kit
Why we built it
Finishing an experiment is only half the work: moving band intensities and Ct values into spreadsheets, normalizing, plotting, building slides and figures, and writing the Methods. WetLab Kit turns that weekly routine into a few minutes.
Tools
Western blot densitometryAutomatic lane and band detection, normalization, replicate statistics, publication figure and slidesqPCR relative quantificationΔΔCt and Pfaffl, multiple reference genes, multi-plate combining, t-tests, chartsFISH signal counterMulti-color spot counting, ratios and HSRs, merges Zeiss / Leica channel filesRNA-seq DEG analysisDEG selection, volcano, heatmap, PCA and Venn from service reports, DESeq2 tables or count matrices; DAVID and GSEA input filesGroup comparison statisticsAutomatic choice of t-test, ANOVA and more, explained in plain words, with graphs showing asterisksIC50 calculatorMTT/CCK-8 viability, 4PL dose–response curves, IC50 with 95% CIStandard curve calculatorBCA, Bradford and ELISA sample concentrations, western blot loading volumesReagent calculatorMolarity and mass, C1V1 dilution, unit conversion, serial dilution, drug stocks, primers and DNA, cell counting and seedingSurvival analysisKaplan-Meier curves, median survival, log-rank test, hazard ratiosMicrobiome 16SRelative abundance, alpha/beta diversity, PCoA and PERMANOVA, and differential taxa from QIIME2, ASV or MetaPhlAn tablesPrimer design & checkPrimer suggestions for qPCR, PCR and cloning; Tm, GC, dimer and hairpin checks; Primer-BLAST linkCloning helperRestriction maps and virtual digests, restriction and Gibson/In-Fusion primers, reverse complement, translation and ORFsRun NGS on your computerRun scripts and install steps to analyse FASTQ on your own PC with nf-core (RNA-seq, 16S)
Principles
- Free, no install or sign-up — all you need is a web browser.
- Your data stays in your browser — files and results are never uploaded or stored, and the site’s security policy blocks outgoing transfers.
- Transparent methods — every tool lists its formulas and statistics, and they appear in the generated Methods text.
- Easy — recommended settings are chosen for your data and the key result comes first.
Please review all results before use. WetLab Kit assists analysis; interpretation and responsibility remain with the researcher.
Contact
Report bugs, ideas or feature requests through the feedback form. We use your feedback to improve the tools.