Reagent calculator

Molarity ↔ mass · dilution · unit conversion · serial dilution · drug stock · DNA & primers · cell seeding — with every step shown
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Mass needed for a molar solution

Questions like "How many mg for 50 mL of 10 mM NaCl?". Mass = concentration × volume × molecular weight. Choose what to solve for.

Solve for:
The same reagent can have different MWs depending on hydrate (·2H₂O) or salt (HCl) form. Check the FW (formula weight) on the bottle label.

Dilution: C1 × V1 = C2 × V2

Works out how much concentrated stock to add to reach the concentration you want. Both concentrations must be the same kind of unit (e.g. mM and µM, or 10× and 1×).

Solve for:

Convert concentration units

Converting µg/mL to µM (or back) needs the molecular weight. Enter one value to see it in every unit.

Serial dilution

Used to make standard curves or IC50 concentration series. Make the first tube, then repeatedly transfer a fixed volume into the next tube (which already holds diluent).

Drug stock and treatment volume

Dissolve a powdered drug in DMSO (or another solvent) to make a stock, then work out how much to add to the medium for the concentration you want.

Adding to medium

Resuspending primers

Uses the nmol value printed on the primer tube to work out how much water (or TE) makes a 100 µM stock.

DNA amount: µg ↔ pmol · copy number

Ligation: insert amount (vector : insert molar ratio)

Concentration from absorbance (A260)

Counting cells with a hemocytometer

Mix with trypan blue and count cells in the four large corner squares (1 mm² each). Record live cells (clear) and dead cells (blue) separately.

Seeding: amount per well