Enter the sample groups (sample name · group, one per line). If left empty, a group column in the table or the sample names (e.g. Ctrl_1, Ctrl_2 → Ctrl) are used automatically.
Taxonomic level
Show top · reference group
Relative abundance (stacked bars)
Alpha diversity (diversity within a sample)
How to read · Observed = number of taxa detected. Shannon = richness and evenness combined (higher = more diverse). Simpson = probability that two random reads belong to different taxa. Groups are compared with rank-based tests (Mann–Whitney or Kruskal–Wallis).
Beta diversity (how different samples are) · Bray–Curtis PCoA
How to read · One dot = one sample; closer dots have more similar communities. The % on each axis is how much of the between-sample variation it explains. PERMANOVA tests whether groups explain community differences using 999 permutations; R² is the fraction of variation explained by group.
Differentially abundant taxa
Rank-based tests on relative abundance (%), corrected for multiple testing with Benjamini–Hochberg (FDR). Intended for a quick look; for publication, confirm with compositional methods such as ANCOM-BC, ALDEx2 or MaAsLin2.