Dilution: "x5", "1:5" or "5x" are all recognized. Without one, the sample is treated as undiluted.
Standard curve
Sample concentrations
Back-calculated standards (how well the curve fits)
If the concentration calculated back from the curve is 80–120% of the real value, the curve fits well.
Western blot loading calculator
Load µg per lane · final volume µL · sample buffer
If the sample volume does not fit (too dilute), load fewer µg or increase the final volume. Loading the same µg in every lane matters most, before loading-control normalization.