① Set up hereTell us the analysis type and FASTQ file names. File contents are never read.

② Run on your PCOne command runs a validated open pipeline (nf-core) on your own CPU. Your data never leaves the computer.

③ Bring results backUpload the result table (gene counts or taxa table) to a WetLab Kit tool for DEG, volcano plots or diversity analysis.

Why not in the browser? Aligning and quantifying FASTQ needs tens of GB of memory and hours of computing — more than one browser tab can offer. Running dedicated software on each person's own computer uses all CPU cores and needs no server upload. The pipelines are the open-source nf-core pipelines (pinned versions) used by labs worldwide.

1Analysis type

RNA-seq gene expressionFASTQ → Salmon → gene count table (nf-core/rnaseq) 16S amplicon microbial communityFASTQ → DADA2 ASVs → SILVA taxonomy (nf-core/ampliseq) Variant calling (WES/WGS) — coming soonNVIDIA GPU acceleration (Parabricks) planned

💡 Just want a quick FASTQ quality check? Drop FASTQ files into the RNA-seq tool for an in-browser quality report (including Illumina/MGI detection) — no install.

2Your computer

Operating system CPU cores · memory (RAM) GB

3Data

Species

Folder with the FASTQ files

Drop FASTQ files here or click to choose
Only the file names are read to build the sample table (contents are not read or sent anywhere). Select all FASTQ files in the folder.

Or paste file names

Edit sample names directly in the table. Files from several lanes of one sample (L001, L002…) are merged automatically.

Run NGS on your computer

1Analysis type

2Your computer

3Data

4Install (first time only)

5Run

6Bring results into WetLab Kit