10% APS (ammonium persulfate) recipe
The initiator that polymerises acrylamide gels; together with TEMED it starts setting within minutes.
🧮 Volume calculator
| Component | Amount | Final concentration (1X) |
|---|---|---|
| Ammonium persulfate (APS) | 1 g | 10% (w/v) |
| Water (distilled) | to final volume |
Steps
- Dissolve APS in water (e.g. 1 g made up to 10 mL).
- Aliquot and freeze at −20 °C, or keep at 4 °C and use within a week.
Good to know
- Old APS is the most common reason gels fail to set.
FAQ
My gel will not set.
Make fresh APS and check that your TEMED is not old.
This is a widely used standard composition. Amounts depend on the hydrate form and purity of your reagents — check the formula weight (FW) on the bottle and follow your institution's safety rules and the manufacturers' instructions.