10% SDS recipe
An anionic detergent stock for SDS-PAGE gels, buffers and cell lysis.
🧮 Volume calculator
| Component | Amount | Final concentration (1X) |
|---|---|---|
| SDS (sodium dodecyl sulfate) | 10 g | 10% (w/v) |
| Water (distilled) | to final volume |
Steps
- Add SDS slowly to about 90% of the final volume (stir gently to avoid foam).
- Warming to about 68 °C speeds up dissolving.
- Bring to volume and store at room temperature.
Good to know
- Do not autoclave. It precipitates in the cold — warm to redissolve.
⚠ Safety Avoid inhaling SDS powder — weigh it with a mask or in a fume hood.
FAQ
My 10% SDS turned cloudy.
It crystallised in the cold. Warm to 37 °C to redissolve.
This is a widely used standard composition. Amounts depend on the hydrate form and purity of your reagents — check the formula weight (FW) on the bottle and follow your institution's safety rules and the manufacturers' instructions.