10% Triton X-100 recipe
Pre-diluting viscous Triton X-100 to 10% makes lysis and permeabilisation buffers faster and more accurate.
🧮 Volume calculator
| Component | Amount | Final concentration (1X) |
|---|---|---|
| Triton X-100 | 5 mL | 10% (v/v) |
| Water (distilled) | to final volume |
Steps
- Measure Triton X-100 with a cut tip or by weight (10.7 g, density 1.07).
- Add water and mix gently by inversion until dissolved (avoid foam).
- Store protected from light at room temperature or 4 °C.
Good to know
- Triton accumulates peroxides with age — prefer fresh bottles.
FAQ
Is Triton X-100 the same as NP-40?
Both are similar non-ionic detergents but not identical. Original NP-40 is discontinued; IGEPAL CA-630 is the usual substitute.
This is a widely used standard composition. Amounts depend on the hydrate form and purity of your reagents — check the formula weight (FW) on the bottle and follow your institution's safety rules and the manufacturers' instructions.