Puromycin 10 mg/mL stock recipe
A selection antibiotic stock for keeping only cells carrying the puromycin-resistance gene. Typically 0.5–10 µg/mL final — set by a kill curve for each cell line.
🧮 Volume calculator
| Component | Amount | Final concentration (1X) |
|---|---|---|
| Puromycin dihydrochloride | 100 mg | 10 mg/mL |
| Water (distilled) | to final volume |
Steps
- Dissolve in water and bring to volume.
- Filter-sterilise through 0.22 µm. Aliquot and store at −20 °C.
Good to know
- Kill curve: choose the lowest dose that kills all untransduced cells within 2–4 days.
⚠ Safety Toxic — wear gloves.
FAQ
How does it compare with G418 or hygromycin?
Puromycin selects in 2–4 days; G418 and hygromycin take 1–2 weeks. Choose by the resistance gene on your vector.
This is a widely used standard composition. Amounts depend on the hydrate form and purity of your reagents — check the formula weight (FW) on the bottle and follow your institution's safety rules and the manufacturers' instructions.