Polybrene 8 mg/mL (hexadimethrine bromide) recipe
A cationic polymer that boosts retro/lentiviral transduction; use at 4–8 µg/mL (1:1000–1:2000).
🧮 Volume calculator
| Component | Amount | Final concentration (1X) |
|---|---|---|
| Hexadimethrine bromide | 80 mg | 8 mg/mL |
| Water (distilled) | to final volume |
Steps
- Dissolve in water and bring to volume.
- Filter-sterilise through 0.22 µm. Aliquot and store at −20 °C.
Good to know
- It can be toxic to primary or stem cells — lower the dose or omit it.
FAQ
When do I remove it?
Usually replace the medium 12–24 h after transduction.
This is a widely used standard composition. Amounts depend on the hydrate form and purity of your reagents — check the formula weight (FW) on the bottle and follow your institution's safety rules and the manufacturers' instructions.