1 M MgCl₂ recipe
A magnesium stock for PCR and enzyme reactions.
🧮 Volume calculator
| Component | Amount | Final concentration (1X) |
|---|---|---|
| MgCl₂·6H₂O | 20.33 g | 1 M |
| Water (distilled) | to final volume |
Steps
- Dissolve MgCl₂·6H₂O in about 80% of the final volume.
- Bring to the final volume.
- Autoclave or filter through 0.22 µm.
Good to know
- MgCl₂ is hygroscopic — close the bottle promptly and use a fresh bottle when accuracy matters.
FAQ
Can I use anhydrous MgCl₂?
Yes — 95.2 g/L (MW 95.21), added slowly because it releases a lot of heat.
This is a widely used standard composition. Amounts depend on the hydrate form and purity of your reagents — check the formula weight (FW) on the bottle and follow your institution's safety rules and the manufacturers' instructions.