0.5 M Tris-HCl pH 6.8 (stacking gel buffer) recipe
The buffer for the SDS-PAGE stacking (upper) gel.
🧮 Volume calculator
| Component | Amount | Final concentration (1X) |
|---|---|---|
| Tris base | 15.14 g | 0.5 M |
| Concentrated HCl | as needed | to pH 6.8 |
| Water (distilled) | to final volume |
Steps
- Dissolve Tris base in about 70% of the final volume of water.
- Adjust to pH 6.8 with HCl (it takes quite a lot).
- Bring to the final volume with water.
- Filter and store at 4 °C.
FAQ
Can I use 1 M Tris pH 6.8 instead?
Yes — use half the volume and add the difference as water; the final concentration (125 mM) is the same.
This is a widely used standard composition. Amounts depend on the hydrate form and purity of your reagents — check the formula weight (FW) on the bottle and follow your institution's safety rules and the manufacturers' instructions.