Southern blot neutralisation solution recipe
Brings the denatured gel back to neutral pH before transfer: 1.5 M NaCl, 0.5 M Tris-HCl pH 7.5.
🧮 Volume calculator
| Component | Amount | Final concentration (1X) |
|---|---|---|
| NaCl | 87.66 g | 1.5 M |
| Tris base | 60.57 g | 0.5 M |
| Concentrated HCl | as needed | to pH 7.5 |
| Water (distilled) | to final volume |
Steps
- Dissolve in about 80% of the final volume of water.
- Adjust to pH 7.5 with HCl.
- Bring to the final volume with water.
- Soak the gel 2 × 15 min, then transfer with 20X SSC.
FAQ
Is EDTA sometimes added?
Yes — a 1 mM EDTA variant is common.
This is a widely used standard composition. Amounts depend on the hydrate form and purity of your reagents — check the formula weight (FW) on the bottle and follow your institution's safety rules and the manufacturers' instructions.