PBST (PBS + 0.1% Tween-20) recipe
A PBS-based wash buffer widely used for ELISA and immunostaining.
🧮 Volume calculator
| Component | Amount | Final concentration (1X) |
|---|---|---|
| 10X PBS | 100 mL | 1X |
| Tween-20 | 1 mL | 0.1% (v/v) |
| Water (distilled) | to final volume |
Steps
- Add 10X PBS to about 800 mL of water.
- Add Tween-20, bring to volume and mix.
Good to know
- ELISA washes often use 0.05% Tween-20 (0.5 mL/L).
FAQ
PBST or TBST?
Both usually work; use TBST when phosphate interferes (AP enzyme, some phospho-antibodies).
This is a widely used standard composition. Amounts depend on the hydrate form and purity of your reagents — check the formula weight (FW) on the bottle and follow your institution's safety rules and the manufacturers' instructions.