10X MOPS buffer (for RNA gels) recipe
The running buffer for formaldehyde RNA gels. 10X = 0.2 M MOPS, 50 mM sodium acetate, 10 mM EDTA, pH 7.0.
🧮 Volume calculator
| Component | Amount | Final concentration (1X) |
|---|---|---|
| MOPS (free acid) | 20.93 g | 0.2 M (10X) |
| Sodium acetate (anhydrous) | 2.05 g | 50 mM (10X) |
| 0.5 M EDTA (pH 8.0) | 10 mL | 10 mM (10X) |
| Water (distilled) | to final volume |
Steps
- Dissolve in DEPC-treated water (about 80% of the final volume).
- Adjust to pH 7.0 with NaOH and bring to volume.
- Filter and store at room temperature in the dark.
Good to know
- It yellows with light; pale yellow is fine, dark brown should be discarded.
FAQ
Do RNA gels have to be denaturing?
For accurate sizing or Northerns, yes. For a quick integrity check, a clean TAE/TBE gel still shows the 28S and 18S bands.
This is a widely used standard composition. Amounts depend on the hydrate form and purity of your reagents — check the formula weight (FW) on the bottle and follow your institution's safety rules and the manufacturers' instructions.