HotSHOT neutralisation reagent recipe
Added in an equal volume after HotSHOT alkaline lysis to bring the pH back: 40 mM Tris-HCl, pH 5.0.
🧮 Volume calculator
| Component | Amount | Final concentration (1X) |
|---|---|---|
| Tris-HCl (Tris hydrochloride) | 630 mg | 40 mM |
| Water (distilled) | to final volume |
Steps
- Dissolve in about 80% of the final volume of water.
- Check pH 5.0 and adjust with HCl if needed.
- Bring to the final volume with water.
- Store at room temperature.
FAQ
Can I make it from Tris base?
Yes — dissolve 4.85 g/L Tris base and titrate to pH 5.0 with HCl.
This is a widely used standard composition. Amounts depend on the hydrate form and purity of your reagents — check the formula weight (FW) on the bottle and follow your institution's safety rules and the manufacturers' instructions.