Chloramphenicol 34 mg/mL (1000X) recipe
Antibiotic stock for cam-resistance plasmids (e.g. pLysS); dissolved in ethanol and used 1:1000 (34 µg/mL).
🧮 Volume calculator
| Component | Amount | Final concentration (1X) |
|---|---|---|
| Chloramphenicol | 340 mg | 34 mg/mL |
| Absolute ethanol | to final volume |
Steps
- Dissolve in the solvent and bring to volume (alcoholic solutions need no filtering).
- Aliquot and store at −20 °C protected from light.
- Add 1 mL per litre (1000X): 34 µg/mL final.
Good to know
- Some protocols use 25 µg/mL.
FAQ
Can I dissolve it in water?
It is poorly soluble in water (≈2.5 mg/mL), so concentrated stocks are made in ethanol.
This is a widely used standard composition. Amounts depend on the hydrate form and purity of your reagents — check the formula weight (FW) on the bottle and follow your institution's safety rules and the manufacturers' instructions.