2xYT medium recipe
A richer-than-LB medium used for phage display, protein expression and M13 phage growth.
🧮 Volume calculator
| Component | Amount | Final concentration (1X) |
|---|---|---|
| Tryptone | 8 g | 1.6% |
| Yeast extract | 5 g | 1% |
| NaCl | 2.5 g | 0.5% |
| Water (distilled) | to final volume |
Steps
- Dissolve in about 80% of the final volume of water.
- Adjust to pH 7.0.
- Bring to the final volume with water.
- Autoclave (121 °C, 20 min). Store at room temperature.
FAQ
How do I make plates?
Add 15 g/L agar before autoclaving.
This is a widely used standard composition. Amounts depend on the hydrate form and purity of your reagents — check the formula weight (FW) on the bottle and follow your institution's safety rules and the manufacturers' instructions.